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| Status |
Public on Feb 25, 2013 |
| Title |
Profiling A375P melanoma cells following PGC1a suppression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
PGC1a is a transcriptional coactivator that regulates energy metabolism. PGC1a is highly expressed in a subset of melanoma tumors and cell lines. We generated gene-expression profile of control and PGC1alpha depleted A375P melanoma cells, a melanoma cell line that expresses very high levels of PGC1a to investigate the role of this gene in melanoma.
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| Overall design |
Lentivirus were used to generate stable cell lines expressing two shRNAs against PGC1alpha or two shRNAs controls. Cells were selected with purmycin for 48h and RNA was harvested 48h after selection and gene-expression arrays were generated.
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| Contributor(s) |
Vazquez F, Puigserver P |
| Citation(s) |
23416000 |
| |
| Submission date |
Mar 28, 2012 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Pere Puigserver |
| E-mail(s) |
pere_puigserver@dfci.harvard.edu
|
| Phone |
617-582-7977
|
| Organization name |
Dana-Farber Cancer Institute
|
| Street address |
450 Brookline Av. CLSB-11144
|
| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA157003 |
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