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Series GSE37159 Query DataSets for GSE37159
Status Public on Jul 08, 2012
Title Interaction between BZR1 and PIF4 integrates brassinosteroid and environmental responses [RNA-seq]
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Plant growth is coordinately regulated by environmental and hormonal signals. Brassinosteroid (BR) plays essential roles in growth regulation by light and temperature, but the interactions between BR and these environmental signals remain poorly understood at the molecular level. Here, we show that direct interaction between the dark- and heat-activated transcription factor phytochrome-interacting factor4 (PIF4) and the BR-activated transcription factor BZR1 integrates the hormonal and environmental signals. BZR1 and PIF4 interact with each other in vitro and in vivo, bind to nearly two thousand common target genes, and synergistically regulate many of these target genes, including the PRE family HLH factors required for promoting cell elongation. Genetic analysis indicates that BZR1 and PIFs are interdependent in promoting cell elongation in response to BR, darkness, or heat. These results show that the BZR1-PIF4 interaction controls a core transcription network, allowing plant growth co-regulation by the steroid and environmental signals.
 
Overall design RNA-Seq for Col-0, bzr1-1D, pifq and pifq;bzr1-1D seedlings grown on BRZ-containing medium in the dark.
 
Contributor(s) Oh E, Wang Z
Citation(s) 22820378
Submission date Apr 11, 2012
Last update date May 15, 2019
Contact name Eunkyoo Oh
Organization name Carnegie Institution
Department Plant Biology
Street address 260 Panama Street
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL13222 Illumina HiSeq 2000 (Arabidopsis thaliana)
Samples (8)
GSM912634 Col-0 RNA-Seq replicate 1
GSM912635 Col-0 RNA-Seq replicate 2
GSM912636 bzr1-1D RNA-Seq replicate 1
This SubSeries is part of SuperSeries:
GSE37160 Interaction between BZR1 and PIF4 integrates brassinosteroid and environmental responses
Relations
BioProject PRJNA158647
SRA SRP012153

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37159_RAW.tar 1.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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