|
| Status |
Public on Nov 14, 2012 |
| Title |
Expression data from WT and VAChT KDHOM ventricles |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
VAChT KDHOM mice have a 70% decrease in the vesicular acetylcholine transporter (VAChT) and this leads to a systemic decrease in ACh release and cardiac dysfunction.
We used this microarray to determine whether there were transcriptional alterations in the hearts of mutant mice as a result of this long-term decrease in cholinergic tone.
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| |
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| Overall design |
We isolated whole hearts from 3-month-old WT and VAChT KD mice and used them for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogenous populations for each sample and, as such, pooled together two RNA samples to generate one microarray sample.
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| |
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| Contributor(s) |
Marco A.M. P, Robert G |
| Citation(s) |
22768193 |
| |
| Submission date |
Apr 20, 2012 |
| Last update date |
Mar 04, 2019 |
| Contact name |
David E Carter |
| E-mail(s) |
dcarter@robarts.ca
|
| Organization name |
Robarts Research Institute
|
| Department |
London Regional Genomics Centre
|
| Street address |
1151 Richmond Street North
|
| City |
London |
| State/province |
ON |
| ZIP/Postal code |
N6A5B7 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (6)
|
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| Relations |
| BioProject |
PRJNA160153 |
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