|
| Status |
Public on May 04, 2012 |
| Title |
Gene expression profile of regulatory T cells (Tregs) isolated from visceral adipose tissue and lymph nodes of mice sufficient and deficient of Pparg expression in Tregs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We identified Pparg as a major orchestrator of the phenotype of adipose-tissue resident regulatory T cells (VAT Tregs). To establish the role of Pparg in shaping the VAT Tregs gene profile and cell dynamics, Tregs from lymph nodes and visceral adipose tissue of mice sufficient and deficient of Pparg expression in Tregs were double sorted for microarray analysis.
|
| |
|
| Overall design |
All gene expression profiles were obtained from highly purified (double-sorted) T cell populations sorted by flow cytometry. To reduce variability, cells from multiple mice were pooled. Triplicates were generated for all groups. Raw data were preprocessed with the RMA algorithm in GenePattern and averaged expression values were used for analysis.
|
| |
|
| Contributor(s) |
Cipolletta D, Mathis D, Benoist C |
| Citation(s) |
22722857, 25550516 |
| |
| Submission date |
Apr 23, 2012 |
| Last update date |
Mar 04, 2019 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (24)
|
|
| This SubSeries is part of SuperSeries: |
| GSE37535 |
PPARĪ³ is a major driver of the accumulation and phenotype of adipose-tissue Treg cells |
|
| Relations |
| BioProject |
PRJNA161103 |
Less...
More...