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Series GSE3757

Query DataSets for GSE3757

Status

Public on May 23, 2006

Title

Gene Expression Profile Signatures Indicate a Role for Wnt Signaling in Endothelial Commtment From Embryonic Stem Cells

Organism

Mus musculus

Experiment type

Expression profiling by array

Summary

Embryonic stem (ES) cells have the potential to generate a variety of cell
lineages including endothelial cells, blood cells and smooth muscle cells.
flk1-expressing cells derived from ES cells serve as vascular progenitors.
We have used global gene expression analysis in order to establish a
comprehensive list of candidate genes in the developing vasculature during
ES cell differentiation in vitro. A large set of genes, including growth
factors, cell surface molecules, transcriptional factors, and members of
several signal transduction pathways that are known to be involved in
vasculogenesis or angiogenesis, were found to have expression patterns as
expected. Some unknown or functionally uncharacterized genes were
differentially regulated in flk1+ cells compared with flk1 cells,
suggesting possible roles for these genes in vascular commitment.
Particularly, multiple components of the Wnt signaling pathway were
differentially regulated in flk1+ cells, including Wnt proteins, their
receptors, downstream transcriptional factors, and other components
belonging to this pathway. Activation of the Wnt signal was able to expand
vascular progenitor populations whereas suppression of Wnt activity reduced
flk1+ populations. Suppression of Wnt signaling also inhibited the formation
of matured vascular capillary-like structures during late stages of EB
differentiation. These data indicate a requisite and ongoing role for Wnt
activity during vascular development, and the gene expression profiles
identify candidate components of this pathway that participate in vascular
cell differentiation.
Keywords: Time course, development, endothelial cell, angiogenesis, embryonic stem cells, mouse, vasculature, Wnt signaling

Overall design

Flk1 positive and negative cells isolated from murine embryoid bodies were subjected to gene expression profiling at 84 hours, 95 hours and eight days post differentiation using the Agilent mouse development platform.

Contributor(s)

Wang H, Charles PC, Wu Y, Ren R, Rubin J, Perou C, Bautch V, Patterson C

Citation(s)

16601226, 17636018

Submission date

Dec 06, 2005

Last update date

Jun 06, 2019

Contact name

Monte S Willis

E-mail(s)

monte_willis@med.unc.edu