|
| Status |
Public on Aug 09, 2012 |
| Title |
Profiling of microRNA expression in lung cancer cells with up-regulation of Zeb1 |
| Platform organism |
Homo sapiens |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
The Zeb1 transcriptional repressor plays a key role in metastasis through the down-regulation of genes that are strong inducers of epithelial differentiation and inhibitors of stem-ness. Here we report that Zeb1 controls the expression of numerous oncogenic and tumor suppressive microRNAs (miRs). Zeb1 stimulated pro-migratory cytoskeletal processes by down-regulating miR-34a and activated Rho GTPases through Arhgap1, a Cdc42 GTPase activating protein and novel miR-34a target gene. Poor-prognosis human lung adenocarcinomas were highly enriched in a cytoskeletal gene signature activated by miR-34a down-regulation. These findings suggest that Zeb1 regulates a miR network and drives pro-migratory cytoskeletal processes through miR-34a.
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| |
|
| Overall design |
Microarray-based interrogation of global miR expression changes in a non-metastatic KP cell line (393P) that undergoes EMT and gains invasive and metastatic capabilities following forced Zeb1 expression (393P_Zeb1).
two group comparison
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| |
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| Contributor(s) |
Creighton C, Kurie J, Ahn Y, Gibbons D |
| Citation(s) |
22850877 |
| |
| Submission date |
May 31, 2012 |
| Last update date |
Nov 08, 2012 |
| Contact name |
Chad Creighton |
| E-mail(s) |
creighto@bcm.tmc.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL8733 |
DiscovArray human miRNA 13k array [HDDA] |
|
| Samples (6)
|
| GSM941032 |
393P cells with control vector, sample 1 |
| GSM941033 |
393P cells with control vector, sample 2 |
| GSM941034 |
393P cells with control vector, sample 3 |
| GSM941035 |
393P cells with Zeb1 overexpression, sample 1 |
| GSM941036 |
393P cells with Zeb1 overexpression, sample 2 |
| GSM941037 |
393P cells with Zeb1 overexpression, sample 3 |
|
| Relations |
| BioProject |
PRJNA167760 |
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