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| Status |
Public on Jun 02, 2014 |
| Title |
Global gene expression of Ruegeria pomeroyi DSS-3 during ammonium-limited, ribose phosphate-, methylphosphonate-, or potassium phosphate-excess growth regimes or during potassium phosphate-limited, ammonium-excess regime |
| Organism |
Ruegeria pomeroyi DSS-3 |
| Experiment type |
Expression profiling by array
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| Summary |
We used the previously designed oligonucleotide microarrays (Bürgmann et al., 2007, Environmental Microbiology, 9: 2742-2755) to detect the mRNA transcripts of R. pomeroyi DSS-3 when the cells were cultured under steady-state conditions limited with ammonium (NH4Cl, 0.26 mM) but with an excess of D-ribose-5-phosphate (C5H9Na2O8P*2H2O, 0.5 mM), methylphosphonic acid (CH5PO3, 0.5 mM), or potassium phosphate (KH2PO4, 0.5 mM), or during ammonium excess (NH4Cl, 2.8 mM) but were limited with potassium phosphate (KH2PO4, 9.2 μM).
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| Overall design |
A total of 13 microarray hybridizations were performed: three biological replicates each from ribose phosphate, methylphosphonate, or potassium phosphate excess growth regimes, three biological replicates from potassium phosphate limited growth regime, and one technical replicate for the potassium phosphate excess growth regime. Data for the technical replicates were averaged and combined, resulted in a total of 12 samples.
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| Contributor(s) |
Moran M, Newton RJ, Chan L, Sharma S |
| Citation missing |
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| Submission date |
Jun 15, 2012 |
| Last update date |
Jun 02, 2014 |
| Contact name |
Leong-Keat Chan |
| E-mail(s) |
leongkeatchan@gmail.com
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| Organization name |
University of Georgia
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| Department |
Marine Sciences
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| Lab |
Dr. Mary Ann Moran
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| Street address |
325 Sanford Drive
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| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602 |
| Country |
USA |
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| Platforms (1) |
| GPL4067 |
UGAmamlab_Silicibacter_pomeroyi_DSS-3_8k_v1.0 |
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| Samples (12)
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| GSM948888 |
Steady-state ribose phosphate excess biological rep 1 of 3 |
| GSM948889 |
Steady-state ribose phosphate excess biological rep 2 of 3 |
| GSM948890 |
Steady-state ribose phosphate excess biological rep 3 of 3 |
| GSM948891 |
Steady-state methylphosphonate excess biological rep 1 of 3 |
| GSM948892 |
Steady-state methylphosphonate excess biological rep 2 of 3 |
| GSM948893 |
Steady-state methylphosphonate excess biological rep 3 of 3 |
| GSM948894 |
Steady-state potassium phosphate excess biological rep 1 of 3 |
| GSM948895 |
Steady-state potassium phosphate excess biological rep 2 of 3 |
| GSM948896 |
Steady-state potassium phosphate excess biological rep 3 of 3 |
| GSM948897 |
Steady-state potassium phosphate limited biological rep 1 of 3 |
| GSM948898 |
Steady-state potassium phosphate limited biological rep 2 of 3 |
| GSM948899 |
Steady-state potassium phosphate limited biological rep 3 of 3 |
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| Relations |
| BioProject |
PRJNA168554 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38740_RAW.tar |
16.8 Mb |
(http)(custom) |
TAR (of GPR, TXT) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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