|
| Status |
Public on Mar 05, 2013 |
| Title |
Histone variant, H2B.Z demarcates the AT-rich promoter regions of the Plasmodium falciparum genome |
| Organism |
Plasmodium falciparum 3D7 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Histone variants are key components of the epigenetic code and evolved to perform specific functions in transcriptional regulation, DNA repair, chromosome segregation and other fundamental processes. H2B.Z is a rare, apicomplexan-specific variant of histone H2B. Here we show that in Plasmodium falciparum H2B.Z localises to euchromatic intergenic regions throughout intraerythrocytic development and together with H2A.Z forms a double-variant nucleosomes subtype. These nucleosomes are enriched in promoters over 3’ intergenic regions and their occupancy generally correlates with the strength of the promoter, but not with its temporal activity. Remarkably, H2B.Z occupancy levels exhibit a clear correlation with the base-composition of the underlying DNA, raising the intriguing possibility that the extreme AT-content of the intergenic regions within the Plasmodium genome might be instructive for histone variant deposition. In summary, our data shows that the H2A.Z/H2B.Z double-variant nucleosome demarcates putative regulatory regions of the P. falciparum epigenome and likely provides a scaffold for dynamic regulation of gene expression in this deadly human pathogen.
|
| |
|
| Overall design |
Genome-wide localization of H2B.Z has been studied at three stages of intraerythrocytic development by Illumina sequencing of chromatin-immunoprecipitated and input DNA.
|
| |
|
| Contributor(s) |
Hoeijmakers WA, Salcedo-Amaya AM, Smits AH, Françoijs K, Treeck M, Gilberger T, Stunnenberg HG, Bartfai R |
| Citation(s) |
23320541 |
| |
| Submission date |
Jul 27, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
H.G. Stunnenberg |
| E-mail(s) |
h.stunnenberg@ncmls.ru.nl
|
| Phone |
+31-24-3610520
|
| Organization name |
Radboud University
|
| Department |
Department of Molecular Biology (274)
|
| Street address |
Geert Grooteplein 28
|
| City |
Nijmegen |
| ZIP/Postal code |
6525 GA |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL15857 |
Illumina Genome Analyzer IIx (Plasmodium falciparum 3D7) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA171532 |
| SRA |
SRP014595 |
Less...
More...