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| Status |
Public on May 17, 2013 |
| Title |
Expression data from mouse optic nerve head after elevation in intraocular pressure |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Reactive astrocytes are typically studied in models that cause irreversible mechanical damage to axons, neuronal cell bodies, and glia. We evaluated the response of astrocytes in the optic nerve head to a subtle injury induced by a brief, mild elevation of the intraocular pressure. Astrocytes demonstrated reactive remodeling showing hypertrophy, process retraction and simplification of their shape.
We used microarray to indentify differentially expressed genes and to investigate the molecular mechanisms of astrogliosis in response to this subtle injury.
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| Overall design |
Six- to eight-week old C57Bl/6 male mice were used in this experiment. One eye underwent an elevation in intraocular pressure to 30 mmHg for 1 hour and then allowed to recover for 3 days. The contralateral eye served as a control. Due to the small tissue size of the mouse optic nerve head, two optic nerve heads were pooled together for each microarray chip. We used 10 mice to generate five biological replicates for each condition.
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| Contributor(s) |
Sun D, Qu J, Jakobs TC |
| Citation(s) |
23650091 |
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| Submission date |
Aug 09, 2012 |
| Last update date |
May 04, 2018 |
| Contact name |
Tatjana Jakobs |
| E-mail(s) |
tatjana_jakobs@meei.harvard.edu
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| Phone |
617-912-0205
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| Organization name |
Massachusetts Eye and Ear Infirmary
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| Department |
Howe Laboratory of Ophthalmology
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| Street address |
20 Staniford Street
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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| Samples (10)
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| GSM983248 |
optic nerve head, control, biological rep1 |
| GSM983249 |
optic nerve head, control, biological rep2 |
| GSM983250 |
optic nerve head, control, biological rep3 |
| GSM983251 |
optic nerve head, control, biological rep4 |
| GSM983252 |
optic nerve head, control, biological rep5 |
| GSM983253 |
optic nerve head, high IOP, biological rep1 |
| GSM983254 |
optic nerve head, high IOP, biological rep2 |
| GSM983255 |
optic nerve head, high IOP, biological rep3 |
| GSM983256 |
optic nerve head, high IOP, biological rep4 |
| GSM983257 |
optic nerve head, high IOP, biological rep5 |
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| Relations |
| BioProject |
PRJNA172339 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40009_RAW.tar |
22.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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