|
| Status |
Public on Apr 01, 2014 |
| Title |
microRNA profiles of serum exosomes in healthy controls and colorectal cancer patients-2 |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
MicroRNAs (miRNAs) are intrinsic regulators in the various cellular processes, and their abnormalities are considered to be involved in the onset of human disorders, including cancer. Circulating miRNA is focused as new cancer biomarker however it is regarded that circulating RNA are released not only from tumor but also by various pathways. Recently, exosomes, small membrane vesicles, have been a major interest in cancer research field, because of their unique biological properties. Exosomes are secreted from various cells and the components (Lipids, mRNAs, miRNAs and proteins) reflect origin of the cells secreting them. Identification of exosomal miRNAs from cancer cells is expected to provide useful biomarkers of cancer.
To identify specific exosomal miRNAs as candidate biomarkers for colorectal cancer, we profiled exosomal miRNAs in sera of colon cancer patients (n=88) at various TNM stages (I to IV) and healthy controls (n=11) and selected significantly higher microRNAs in serum exosomes of colorectal cancer patients than that of healthy controls. Moreover, we tried to detect their serum exosome levels of using samples from patients after surgical resection of primary tumors (n=24).
|
| |
|
| Overall design |
Serum exosomes were prepared by step-wise ultra-centrifugation methods in 24 colorectal cancer patients (age; 35-65) after surgical resection of primary tumor (TNM stage I; n=6, stage II; n=5, stage IIIa; n=5, stage IIIb; n=5, stage IV; n=3) .Exosome fraction was mixed with Trizol-LS reagent (Invitrogen), and aqueous phase was collected by adding chloroform. After addition of ethanol to the aqueous phase, it was placed on to an RNeasy mini spin column (Qiagen) for the purification of total RNAs. The total RNAs were analyzed by Agilent Human miRNA V3 Microarray (G4470C) according to the manufacturer's instructions.
|
| |
|
| Contributor(s) |
Tsuchiya N, Ogata-Kawata H |
| Citation(s) |
24705249 |
| |
| Submission date |
Aug 21, 2012 |
| Last update date |
Jan 19, 2017 |
| Contact name |
Naoto Tsuchiya |
| Organization name |
National Cancer Center Reasearch Institute
|
| Lab |
Genome Biology
|
| Street address |
Tsukiji 5-1-1
|
| City |
Chuo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL14767 |
Agilent-021827 Human miRNA Microarray G4470C (Feature Number version) |
|
| Samples (24)
|
|
| This SubSeries is part of SuperSeries: |
| GSE40247 |
Profiling of endogenous and exosomal microRNAs in colon cancer |
|
| Relations |
| BioProject |
PRJNA173337 |
Less...
More...