|
| Status |
Public on Mar 01, 2006 |
| Title |
Yeast Genomic Expression Patterns in Response to Low-Shear Modeled Microgravity |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
The goal of this study was to assess whether low shear-modeled microgravity (LSMMG) effects yeast
genomic expression patterns using the powerful tool of whole genome microarray hybridization. We determined
changes in the yeast model organism, Saccharomyces cerevisisae, when grown in LSMMG using the rotating High
Aspect Ratio Vessel (HARV). A significant number of genes were up- or down-regulated by at least two fold in cells
that were grown for 5 generations or 25 generations in HARVs. We identified genes in cell wall integrity signaling
pathways containing MAP kinase cascades that may provide clues to novel physiological responses of eukaryotic
cells to the external stress of a low-shear modeled microgravity environment. A comparison of the microgravity
response to other environmental stress response (ESR) genes showed that 26% of the genes that respond
significantly to LSMMG are involved in a general environmental stress response, while 74% of the genes may
represent a unique transcriptional response to microgravity. In addition, we found changes in genes involved in
budding, cell polarity establishment, and cell separation that confirm our hypothesis that exposure to LSMMG
causes changes in gene transcription resulting in a phenotypic response. The results of the study provide interesting
clues to potential mechanisms involved in the response to, adaptation to, and survival of eukaryotic cells in a
microgravity environment and our findings may have important health implications for human spaceflight.
Keywords: time course, stress response, budding, microgravity
|
| |
|
| Overall design |
Four conditions are compared with three replicates each: yeast grown in low-shear modeled microgravity (HARV bioreactor) for 5 and 25 generations; yeast grown in a horizontal (non-LSMMG) HARV bioreactor for 5 and 25 generations.
|
| |
|
| Contributor(s) |
Sheehan KB, McInnerney K, Purevdorj-Gage BL, Hyman LE |
| Citation(s) |
17201921 |
| |
| Submission date |
Jan 31, 2006 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Kate McInnerney |
| E-mail(s) |
kmcinnerney@montana.edu
|
| Phone |
406-994-5666
|
| Fax |
406-994-4926
|
| URL |
http://cores.montana.edu
|
| Organization name |
Montana State University
|
| Department |
Microbiology
|
| Lab |
Functional Genomics Facility
|
| Street address |
109 Lewis Hall
|
| City |
Bozeman |
| State/province |
MT |
| ZIP/Postal code |
59717 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
|
| Samples (12)
|
| GSM94606 |
Control sample 5th generation_rep1 |
| GSM94608 |
Control sample 5th generation_rep2 |
| GSM94609 |
Control sample 5th generation_rep3 |
| GSM94610 |
Control sample 25th generation_rep1 |
| GSM94611 |
Control sample 25th generation_rep2 |
| GSM94612 |
Control sample 25th generation_rep3 |
| GSM94613 |
Low shear modeled microgravity sample 5th generation_rep1 |
| GSM94614 |
Low shear modeled microgravity sample 5th generation_rep2 |
| GSM94615 |
Low shear modeled microgravity sample 5th generation_rep3 |
| GSM94616 |
Low shear modeled microgravity sample 25th generation_rep1 |
| GSM94617 |
Low shear modeled microgravity sample 25th generation_rep2 |
| GSM94618 |
Low shear modeled microgravity sample 25th generation_rep3 |
|
| Relations |
| BioProject |
PRJNA95019 |
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