|
| Status |
Public on Nov 01, 2012 |
| Title |
Female Mice Lacking p47phox Have Altered Adipose Tissue Gene Expression and are Protected against High Fat-Induced Obesity and Metabolic Syndrome |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Oxidative stress in adipose tissue and liver has been linked to the development of obesity. NADPH oxidases (NOX) enzymes are a major source of reactive oxygen species (ROS). The current study was designed to determine if NOX2-generated ROS play a role in development of obesity and metabolic syndrome after high fat feeding. Wild type (WT) mice and mice lacking the cytosolic NOX2 activated protein p47phox (P47KO) were fed AIN-93G diets or high fat diets (HFD) containing 45% fat and 0.5% cholesterol for 13 weeks from weaning. Affymetrix array analysis revealed dramatically less expression of mRNA of genes linked to energy metabolism, adipocyte differentiation (PPARĪ³, Runx2) and fatty acid uptake (CD36, lipoprotein lipase) in fat pads from female HFD-P47KO mice compared to HFD-WT females. These data suggest that NOX2 is an important regulator of metabolic homeostasis and that NOX2-associated ROS plays an important role in development of diet-induced obesity particularly in the female
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| |
|
| Overall design |
fat pads from p47phox and wild type fed a high fat or control diet
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| |
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| Contributor(s) |
Ronis MJ, Sharma N, Vantrease J, Borengasser SJ, Ferguson M, Gomez-Acevedo H, Badger TM |
| Citation(s) |
23482812 |
| |
| Submission date |
Oct 31, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Horacio Gomez-Acevedo |
| E-mail(s) |
horacio.g.acevedo@gmail.com
|
| Organization name |
University of Arkansas for Medical Sciences
|
| Department |
Biomedical Informatics
|
| Street address |
4301 W. Markham St
|
| City |
Little Rock |
| State/province |
Arkansas |
| ZIP/Postal code |
72205 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA178540 |
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