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| Status |
Public on Nov 08, 2012 |
| Title |
Expression data from Leishmania major infected human dendritic cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Leishmania major infected human dendritic cells (DCs) exhibit a marked induction of IL-12 ultimately promoting a robust Th1-mediated response associated with parasite killing and protective immunity. In this study, we utilized Affymetrix Genechips to globally assess the host cell genes and pathways associated with L. major infection during early infection (2, 4, 8, and 24 hrs) in human myeloid-derived DCs. Bioinformatic analyses of the hybridized microarray chips identified 728 genes, represented by 848 unique probe sets, which, when compared to uninfected samples were observed to be significantly differentially expressed by one-way ANOVA. Altogether, the data provide a genome-wide perspective on the transcriptional influences Leishmania species exert within human DCs during early infection, and provides a platform for further investigations toward functionally characterizing candidate genes of importance to the IL-12 based immune response to infections.
In the current study, we further investigate the L. major infected DC transcriptional during early time points after infection via microarray analysis.
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| Overall design |
The cRNAs for each L. major infected time point (2, 4, 8, 24 hrs) including an uninfected sample (2 hrs), from three individual donors, were extracted and hybridized to 15 individual human HG-U133 Plus2.0 Affymetrix microarray gene chips, whereby each chip represented the expression profile for one donor at a given infection time point.
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| Contributor(s) |
Favila MA, McDowell MA |
| Citation(s) |
24808365 |
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| Submission date |
Nov 06, 2012 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Michelle A Favila |
| E-mail(s) |
michellefavila@gmail.com
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| Organization name |
University of Notre Dame
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| Department |
Biology
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| Lab |
Mary Ann McDowell
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| Street address |
210 Galvin Life Science Center
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| City |
Notre Dame |
| State/province |
IN |
| ZIP/Postal code |
46556 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (15)
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| GSM1032235 |
Human Dendritic Cell uninfected 2hrs biological rep1 |
| GSM1032236 |
Human Dendritic Cell uninfected 2hrs biological rep2 |
| GSM1032237 |
Human Dendritic Cell uninfected 2hrs biological rep3 |
| GSM1032238 |
Human Dendritic Cell L. major 2hrs biological rep1 |
| GSM1032239 |
Human Dendritic Cell L. major 2hrs biological rep2 |
| GSM1032240 |
Human Dendritic Cell L. major 2hrs biological rep3 |
| GSM1032241 |
Human Dendritic Cell L. major 4hrs biological rep1 |
| GSM1032242 |
Human Dendritic Cell L. major 4hrs biological rep2 |
| GSM1032243 |
Human Dendritic Cell L. major 4hrs biological rep3 |
| GSM1032244 |
Human Dendritic Cell L. major 8hrs biological rep1 |
| GSM1032245 |
Human Dendritic Cell L. major 8hrs biological rep2 |
| GSM1032246 |
Human Dendritic Cell L. major 8hrs biological rep3 |
| GSM1032247 |
Human Dendritic Cell L. major 24hrs biological rep1 |
| GSM1032248 |
Human Dendritic Cell L. major 24hrs biological rep2 |
| GSM1032249 |
Human Dendritic Cell L. major 24hrs biological rep3 |
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| Relations |
| BioProject |
PRJNA179169 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42088_RAW.tar |
68.1 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE42088_significant_probe_sets.txt.gz |
94.8 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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