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| Status |
Public on Apr 24, 2014 |
| Title |
Compendium of deletion mutant gene expression profiles, 784 non-responsive mutants [hs1990] |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
This dataset consists of 784 non-responsive mutants (three or less significant mRNA expression changes as a result of the deletion) from a gene expression profile compendium of 1,484 deletion mutants that have a role or are implicated in mRNA transcription regulation, mRNA turnover, signaling or are located in the nucleus.
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| Overall design |
Two channel microarrays were used. RNA isolated from a large amount of wt yeast from a single culture was used as a common reference. This common reference was used in one of the channels for each hybridization and used in the statistical analysis to obtain an average expression-profile for each deletion mutant relative to the wt. Two independent cultures were hybridized on two separate microarrays. For the first hybridization the Cy5 (red) labeled cRNA from the deletion mutant is hybridized together with the Cy3 (green) labeled cRNA from the common reference. For the replicate hybridization, the labels are swapped. Each gene is represented twice on the microarray, resulting in four measurements per mutant. Using the Erlenmeyer growth protocol up to five deletion strains were grown on a single day. In the tecan platereader, up to eleven deletion strains could be grown on a single day. Wt cultures were grown parallel to the deletion mutants to assess day-to-day variance.
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| Contributor(s) |
Kemmeren P, Sameith K, van de Pasch L, Holstege F |
| Citation(s) |
24766815, 24952590 |
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| Submission date |
Nov 26, 2012 |
| Last update date |
Nov 07, 2015 |
| Contact name |
Patrick Kemmeren |
| Organization name |
UMC Utrecht
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| Department |
Department of Molecular Cancer Research
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| Lab |
Holstege Lab
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| Street address |
Universiteitsweg 100
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| City |
Utrecht |
| State/province |
Utrecht |
| ZIP/Postal code |
3584 CG |
| Country |
Netherlands |
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| Platforms (1) |
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| Samples (1246)
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| This SubSeries is part of SuperSeries: |
| GSE42528 |
Compendium of deletion mutant gene expression profiles |
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| Relations |
| BioProject |
PRJNA182220 |
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