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| Status |
Public on Jan 01, 2014 |
| Title |
Suppression of prp28-1 splicing defect by prp8-tes allele |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
DEAD-box proteins, a family of RNA-dependent ATPases, promote the numerous conformational rearrangements required for spliceosome assembly, activation, and disassembly. Previous work showed that a cold-sensitive substitution in DEAD-box protein Prp28 prevents the switch from U1 to U6 snRNA pairing with the 5’ splice site. Little is known about how Prp28 is regulated, although U5 snRNP protein Prp8 is a potential coordinator of Prp28 and other spliceosomal ATPases. We conducted a targeted selection in Prp8 for cold-insensitive suppressors of prp28-1, then used splicing specific microarrays to assess suppression of the prp28-1 splicing defect.
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| Overall design |
Splicing specific microarrays were used to assess suppression of the prp28-1 splicing defect by a prp8 allele (prp8-tes) that suppresses prp28-1 cold-sensitivity
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| Contributor(s) |
Price AM, Gornemann J, Guthrie C, Brow DA |
| Citation(s) |
24231520 |
| |
| Submission date |
Dec 05, 2012 |
| Last update date |
Feb 04, 2014 |
| Contact name |
Argenta Price |
| Organization name |
University of California, San Francisco
|
| Lab |
Christine Guthrie
|
| Street address |
600 16th St
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL5052 |
GUTHRIE_YEAST_SPLICINGARRAY_v20040216a |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA183015 |
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