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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 04, 2013 |
| Title |
Human CD1c+ dendritic cells drive the differentiation of CD103+ CD8+ mucosal T cells via TGF-β |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In comparison to murine dendritic cells (DCs), less is known about the function of human DCs in tissues. Here, we analyzed, using lung tissues from humans and humanized mice, the role of human CD1c+ and CD141+ DCs in determining the type of CD8+ T cell immunity to live-attenuated influenza virus (LAIV) vaccine. We found that both lung DC subsets acquired influenza antigens in vivo and expanded specific cytotoxic CD8+ T cells in vitro. However, lung-tissue-resident CD1c+ DCs but not CD141+ DCs were able to drive CD103 expression on CD8+ T cells and promote CD8+ T cell accumulation in lung epithelia in vitro and in vivo. CD1c+ DCs induction of CD103 expression was dependent on membrane-bound TGF-β1. Thus, CD1c+ and CD141+ DCs generate CD8+ T cells with different properties, and CD1c+ DCs specialize in the regulation of mucosal CD8+ T cells.
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| Overall design |
Total RNA were isolated from purified human CD1c+ (BDCA1+) and CD141+ (BDCA3+) mDCs sorted from different tissues, including human blood, spleen and lungs of humanized mice, and human lungs. Eighteen samples in total were analyzed from different donors and tissues.
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| Contributor(s) |
Yu CI, Becker C, Wang Y, Marches F, Helft J, Leboeuf M, Anguiano E, Pourpe S, Goller K, Pascual V, Banchereau J, Merad M, Palucka K |
| Citation(s) |
23562160 |
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| Submission date |
Dec 28, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Chun I Yu |
| E-mail(s) |
chun.yu@baylorhealth.edu
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| Organization name |
Baylor Institute for Immunology Research
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| Street address |
3434 Live Oak St
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| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75204 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA184857 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE43184_RAW.tar |
26.2 Mb |
(http)(custom) |
TAR |
| GSE43184_non-normalized.txt.gz |
5.3 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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