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| Status |
Public on Jan 13, 2014 |
| Title |
Microarray of cardiac biventricle from PGC-1a-/-bf/f/MerCre mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The following abstract from the submitted manuscript describes the major findings of this work.
The metabolic development of high energy-utilizing organs such as the heart involves mitochondrial proliferation at birth followed by a maturation process during the postnatal period. Conditional gene targeting was used in mice to explore the role of the PPARgamma coactivator 1 (PGC-1) coactivators during postnatal development and in adult heart. Marked mitochondrial derangements were observed in hearts of PGC-1a/b-deficient mice during the postnatal period, including fragmentation and elongation associated with the development of a lethal cardiomyopathy. The expression of multiple genes involved in mitochondrial fusion and fission was downregulated in hearts of PGC-1a/b-deficient mice. PGC-la was shown to activate transcription of the mitofusin 1 (Mfn1) gene by coactivating the estrogen-related receptor a (ERRa) upon a highly conserved element. Surprisingly, PGC-1a/b deficiency did not alter cardiac function or general mitochondrial density and myocyte distribution in adult heart. However, transcriptional profiling and mitochondrial function studies demonstrated that the PGC-1 coactivators are required for full respiratory capacity and high level expression of nuclear- and mitochondrial-encoded genes involved in mitochondrial energy transduction and oxidative phosphorylation pathways in adult heart. These results unveil distinct developmental stage-specific transcriptional programs involved in the maturation and maintenance of mitochondria.
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| Overall design |
RNA from five PGC-1a-/- and five PGC-1a-/-bf/f/MerCre mice was analyzed.
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| Contributor(s) |
Lai L, Martin O, Kelly D |
| Citation(s) |
24366168 |
| |
| Submission date |
Jan 28, 2013 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Ola Martin |
| E-mail(s) |
omartin@burnham.org
|
| Phone |
407-745-2000
|
| Organization name |
Sanford-Burnham Medical Research Institute
|
| Lab |
Dr. Dan Kelly
|
| Street address |
6400 Sanger Rd
|
| City |
Orlando |
| State/province |
FL |
| ZIP/Postal code |
32827 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
|
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| Relations |
| BioProject |
PRJNA187450 |
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