|
| Status |
Public on Feb 08, 2013 |
| Title |
Expression data from MCF7 Scrambled and SIM2si cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
MCF7 breast cancer cells are a luminal-type breast cancer with moderate native levels of SIM2s. To determine effects of SIM2s on tumor progression, cells were stably transduced with SIM2si shRNA to knockdown expression, inducing an EMT effect. Microarray analysis was performed to determine genetic pathways involved in this phenotype.
The coordination of cellular metabolism is a key factor in the progression of ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC). Pathways regulating the balance between oxidative phosphorylation and glycolysis are unclear. We have found that transcription factor Singleminded-2s (SIM2s), commonly lost with breast cancer progression, contributes to metabolic regulation by controlling glycolytic flux and cellular senescence. Through promotion of p21 and cellular senescence, SIM2s decreases glycolytic enzyme activity and promotes oxidative phosphorylation in breast cancer. These, coupled with increased autophagy and ROS, inhibit tumor growth and metastasis.
We use microarrays to detail the global gene programming changes that occur with loss of SIM2s gene expression.
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| Overall design |
Scrambled and SIM2si cells were grown in triplicate for RNA extraction and hybridization on Amersham microarrays.
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| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 08, 2013 |
| Last update date |
Oct 28, 2014 |
| Contact name |
Kelly C Scribner |
| E-mail(s) |
kscribner@cvm.tamu.edu
|
| Organization name |
Texas A&M University
|
| Department |
VIBS
|
| Lab |
Weston Porter
|
| Street address |
448 TAMU
|
| City |
College Station |
| State/province |
Texas |
| ZIP/Postal code |
77843 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL2895 |
GE Healthcare/Amersham Biosciences CodeLink Human Whole Genome Bioarray |
|
| Samples (6)
|
| GSM1080504 |
MCF7 Scrambled Controls Cells, Biological Rep 1 |
| GSM1080505 |
MCF7 Scrambled Controls Cells, Biological Rep 2 |
| GSM1080506 |
MCF7 Scrambled Controls Cells, Biological Rep 3 |
|
| Relations |
| BioProject |
PRJNA189047 |
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