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Series GSE44709 Query DataSets for GSE44709
Status Public on Feb 28, 2013
Title Expression data from Bifidobacterium longum strains exposed to hydrogen peroxide stress
Platform organisms Bifidobacterium longum; Bifidobacterium animalis subsp. lactis
Sample organisms Bifidobacterium longum NCC2705; Bifidobacterium longum
Experiment type Expression profiling by array
Summary Stress survival tactics in bacteria utalize the up- and down-regulation of stress response genes. In bacterial that lack classical stress response genes for oxidative stress, other cellular systems can be used for cell survival.
We used custom microarrays to study the regulation of genes in Bifidobacterium longum strains to oxidative stress to elucidate novel stress response mechanisms.
 
Overall design Bifidobacterium cells were grown to late log phase then harvested and exposed to a sub-lethal level of hydrogen peroxide. Samples were taken at 5 and 60 mins for RNA extraction and hybridization on Affymetrix microarrays. Controls were prepared for each time point which recieved no hydrogen peroxide treatment.
 
Contributor(s) Oberg TS, Broadbent JR
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Submission date Feb 27, 2013
Last update date Mar 01, 2013
Contact name Taylor S Oberg
E-mail(s) taylor.oberg@aggiemail.usu.edu
Organization name Utah State University
Department NDFS
Lab Broadbent
Street address 8700 Old Main
City Logan
State/province UT
ZIP/Postal code 84322
Country USA
 
Platforms (1)
GPL16727 [Blactisa520677F] Custom Affymetrix Bifidobacteria Genome Array
Samples (24)
GSM1089199 D2957 unexposed control cells at 0 min, biological rep1
GSM1089200 D2957 unexposed control cells at 0 min, biological rep2
GSM1089201 D2957 unexposed control cells at 0 min, biological rep3
Relations
BioProject PRJNA191072

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44709_RAW.tar 11.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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