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| Status |
Public on Sep 10, 2013 |
| Title |
Dynamic profile of gene expression in rice flag leaf under high temperature |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
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| Summary |
To comprehend the gene expression profile in rice flag leaf under high temperature, Agilent 4×44k rice oligo microarray experiments were carried out using rice flag leaf of reproductive stage at 0 min, 20 min, 60 min, 2 hr, 4 hr, and 8 hr after the treatment of 40 degree centigrade, and the significantly expressed genes mainly involved in transcriptional regulation, transport, protein binding, antioxidant, and stress response were identified. Among them, the predominant transcription factor gene families were Hsf, NAC, AP2/ERF, WRKY, MYB, and C2H2. KMC analysis discovered the time-dependent gene expression pattern under heat. The metabolism pathway analysis demonstrated that, under heat treatment, glycolysis and ubiquitin-proteasome was enhanced, and TCA, gluconeogenesis, the secondary metabolism and light-reaction in the photosystem was dramatically repressed, which revealed the great importance of maintaining primary metabolism and protein homeostasis in response to heat in rice flag leaf.
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| Overall design |
Heat shock induced gene expression in rice flag leaf of reproductive stage was measured at 0 min, 20 min, 60 min, 2 hr, 4 hr, and 8 hr after the treatment of 40 degree centigrade in plant growth chamber. Two independent replicate experiments were performed at each time point.
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| Contributor(s) |
Zhang X, Li J, Liu A, Zou J, Chen X |
| Citation missing |
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| Submission date |
Mar 18, 2013 |
| Last update date |
Jan 09, 2014 |
| Contact name |
Xianwen Zhang |
| E-mail(s) |
zhangxianwen@yahoo.com.cn
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| Organization name |
Hunan Agricultural University
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| Street address |
1 Nongda Road, Furong District
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| City |
Changsha |
| State/province |
Hunan |
| ZIP/Postal code |
410128 |
| Country |
China |
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| Platforms (1) |
| GPL8852 |
Agilent-015241 Rice Gene Expression 4x44K Microarray (Probe name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA193327 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE45259_RAW.tar |
92.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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