|
| Status |
Public on Jun 01, 2013 |
| Title |
Distinct translational control in CD4+ T-cell subsets |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Regulatory T cells expressing the transcription factor Foxp3 play indispensable roles for the induction and maintenance of immunological self-tolerance and immune homeostasis. Genome-wide mRNA expression-studies have defined canonical signatures of T-cell subsets. Changes in steady-state mRNA levels do, however, often not reflect those of corresponding proteins due to post-transcriptional mechanisms including mRNA translation. Here, we unveil a unique translational signature, contrasting CD4+Foxp3+ regulatory T (TFoxp3+) and CD4+Foxp3- non-regulatory T (TFoxp3-) cells, which imprints subset-specific protein expression. We further show that translation of eukaryotic translation initiation factor 4E (eIF4E) is induced during T-cell activation and, in turn, regulates translation of cell cycle related mRNAs and proliferation in both TFoxp3- and TFoxp3+ cells. Unexpectedly, eIF4E also affects Foxp3 expression and thereby lineage identity. Thus, mRNA-specific translational control directs both common and distinct cellular processes in CD4+ T-cell subset.
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| |
|
| Overall design |
CD4+/Foxp3+ and CD4+Foxp3- cells were studied ex vivo or activated in vitro for 36h. Both polysome-associated and cytoplasmic RNA was isolated to enables studies of translational control
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| |
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| Contributor(s) |
Larsson O |
| Citation(s) |
23658533 |
| |
| Submission date |
Mar 21, 2013 |
| Last update date |
Aug 20, 2013 |
| Contact name |
Ola Larsson |
| E-mail(s) |
ola.larsson@ki.se
|
| Phone |
+46 (0)8 517 73280
|
| Organization name |
Karolinska Institutet
|
| Department |
Department of oncology-pathology
|
| Street address |
CCK, R8:01
|
| City |
Stockholm |
| ZIP/Postal code |
171 76 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL7546 |
Affymetrix GeneChip Mouse Genome 430 2.0 Array [CDF: Mm_ENTREZG_10] |
|
| Samples (16)
|
| GSM1103576 |
Foxp3- ex vivo_Polysome-associated RNA_4 |
| GSM1103577 |
Foxp3+ ex vivo_Cytoplasmic RNA_5 |
| GSM1103578 |
Foxp3+ ex vivo_Polysome-associated RNA_6 |
| GSM1103579 |
Foxp3- ex vivo_Cytoplasmic RNA_7 |
| GSM1103580 |
Foxp3- ex vivo_Polysome-associated RNA_8 |
| GSM1103581 |
Foxp3- activated in vitro_Cytoplasmic RNA_9 |
| GSM1103582 |
Foxp3- activated in vitro_Polysome-associated RNA_10 |
| GSM1103583 |
Foxp3- activated in vitro_Cytoplasmic RNA_11 |
| GSM1103584 |
Foxp3- activated in vitro_Polysome-associated RNA_12 |
| GSM1103585 |
Foxp3+ activated in vitro_Cytoplasmic RNA_13 |
| GSM1103586 |
Foxp3+ activated in vitro_Polysome-associated RNA_14 |
| GSM1103587 |
Foxp3+ activated in vitro_Cytoplasmic RNA_15 |
| GSM1103588 |
Foxp3+ activated in vitro_Polysome-associated RNA_16 |
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| Relations |
| BioProject |
PRJNA193669 |
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