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| Status |
Public on Jun 22, 2016 |
| Title |
Genome-wide analysis of rats' mRNA profile after knocking down PIWIL1 in embryonic cultured neurons. |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have demonstrated that PIWIL1 can regulate neuronal radial migration during corticogenesis. In order to explore the mechanism, we carried out high-throughtput sequencing (mRNA profile) to define downstream target of PIWIL during the process of neuronal migration. And we found the expression level of several microtubule-associated proteins decreased after downregulation of PIWIL1. Therefore, PIWIL1 plays an important role in neuronal migration by regulating the expression microtubule-associated proteins (such as Map1B, Map2, Tau) .
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| |
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| Overall design |
Examine mRNA profile in two groups of primary cultured neurons with electroporation of control or RNAi plasmid.
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| |
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| Contributor(s) |
Zhao P, Yao M, Chang S, Yuan X |
| Citation(s) |
26104391 |
| |
| Submission date |
Jun 24, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Pingping Zhao |
| E-mail(s) |
ppzhao@ion.ac.cn
|
| Phone |
8613671699349
|
| Organization name |
Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
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| Street address |
No. 320, YueYang Road, Shanghai, China
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| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (2) |
| GSM1173208 |
Culture neuron -control electroporation 48h |
| GSM1173209 |
Culture neuron -RNAi electroporation 48h |
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| Relations |
| BioProject |
PRJNA209323 |
| SRA |
SRP026262 |
