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| Status |
Public on May 05, 2015 |
| Title |
Chromatin remodeling and histone mRNA accumulation in bovine germinal vesicle oocytes |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by array
|
| Summary |
The objective of the study was to compare the transcriptome of the bovine oocyte according to the chromatin configuration of the germinal vesicule
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| |
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| Overall design |
Bovine oocyte (4 biological replicates) were collected and grouped according to the different chromatin configuration of the germinal vesicule described by Lodde et al. 2007. Four GV stages corresponding to the state of chromatin condensation were used (GV0; GV1; GV2 and GV3). Multiples microarray comparison were performed. First a reference design (GV0 as a reference) were used for a total of three comparisons (GV0 vs GV1; GV0 vs GV2 and GV0 vs GV3), then another comparison was performed between the last two groups (GV2 vs GV3). Each comparison was performed using a dye-swap set-up.
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| |
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| Contributor(s) |
Labrecque R, Dieci C, Lodde V, Tessaro I, Luciano AM, Sirard M |
| Citation(s) |
25940597 |
| |
| Submission date |
Jun 27, 2013 |
| Last update date |
Aug 04, 2015 |
| Contact name |
Remi Labrecque |
| E-mail(s) |
remi.labrecque.2@ulaval.ca
|
| Organization name |
Laval University
|
| Department |
Animal Science
|
| Lab |
Genomic and proteomic
|
| Street address |
2440, boulevard Hochelaga
|
| City |
Quebec |
| State/province |
QC |
| ZIP/Postal code |
G1V 0A6 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL13226 |
Agilent-028298 Embryogene Bovine 45K microarray |
|
| Samples (16)
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|
| Relations |
| BioProject |
PRJNA209869 |
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