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Status |
Public on Sep 01, 2013 |
Title |
Gene regulation by Pax6 in the lens placode - E9.5 (1) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Wild type or Pax6 fx/fx; Le-Cre-positive (Pax6-/-) surface ectoderm from E9.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified and reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6 bead arrays.
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Overall design |
Three wild type and three knockout embryos were used. Each array used the cDNA obtained from the two prospective lens tissues (left and right eyes) from each embryo.
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Contributor(s) |
Beebe DC, Huang J |
Citation(s) |
21540023 |
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Submission date |
Jul 25, 2013 |
Last update date |
Sep 23, 2013 |
Contact name |
David Cy Beebe |
E-mail(s) |
beebe@wustl.edu
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Organization name |
Washington University School of Medicine
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Department |
Ophthalmology and Visual Science
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Lab |
Campus Box 8096
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Street address |
660 S. Euclid Ave.
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City |
Saint Louis |
State/province |
MO |
ZIP/Postal code |
63110 |
Country |
USA |
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Platforms (1) |
GPL6105 |
Illumina mouse-6 v1.1 expression beadchip |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE49230 |
Gene regulation by Pax6 in the lens placode - E9.5 |
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Relations |
BioProject |
PRJNA213320 |