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| Status |
Public on Sep 26, 2013 |
| Title |
Expression data from two breast cancer cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
During cancer progression, carcinoma cells encounter a variety of cytotoxic stresses such as hypoxia, nutrient deprivation, and low pH as a result of inadequate vascularization. To maintain survival and growth in the face of these physiologic stressors, a set of adaptive response pathways are induced. One adaptive pathway well studied in other contexts is the unfolded protein response (UPR), of which XBP1 is an important component.
We used microarrays to detect transcriptome profile changes after XBP1 knockdown in breast cancer cell lines, and identify genes and pathways regulated by XBP1, which could help elucidate how XBP1 mediates the adaptive response of breast cancer to cytotoxic stresses.
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| Overall design |
We extracted RNA and hybridized it to Affymetrix microarrays in two breast cancer cell lines (T47D and MDA-MB-231) under treated (hypoxia and glucose deprivation) or untreated conditions with XBP1 knockdown or not.
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| Contributor(s) |
Xi C, Laurie G |
| Citation(s) |
24670641 |
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| Submission date |
Aug 18, 2013 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Xiaole Shirley Liu |
| E-mail(s) |
xsliu.dfci@gmail.com
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| Phone |
(617) 632-2472
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| Organization name |
Dana-Farber Cancer Institute, Harvard School of Public Health
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| Department |
Department of Biostatistics and Computational Biology
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| Street address |
450 Brookline Avenue, CLS 11007
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215-5450 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (8)
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| GSM1210723 |
T47D,shCtrl,Hypoxia+Glucose deprivation,biological rep 1 |
| GSM1210724 |
T47D,shCtrl,Hypoxia+Glucose deprivation,biological rep 2 |
| GSM1210725 |
T47D,shXBP1,Hypoxia+Glucose deprivation,biological rep 1 |
| GSM1210726 |
T47D,shXBP1,Hypoxia+Glucose deprivation,biological rep 2 |
| GSM1210735 |
MDA-MB-231,shCtrl,Hypoxia+Glucose deprivation,biological rep 1 |
| GSM1210736 |
MDA-MB-231,shCtrl,Hypoxia+Glucose deprivation,biological rep 2 |
| GSM1210737 |
MDA-MB-231,shXBP1,Hypoxia+Glucose deprivation,biological rep 1 |
| GSM1210738 |
MDA-MB-231,shXBP1,Hypoxia+Glucose deprivation,biological rep 2 |
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| This SubSeries is part of SuperSeries: |
| GSE49955 |
Genome-wide maps of XBP1 binding sites in different breast cancer cell lines. |
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| Relations |
| BioProject |
PRJNA221364 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE49953_RAW.tar |
41.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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