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| Status |
Public on Oct 08, 2014 |
| Title |
Expression data from Saccharomyces cerevisiae strains deleted for the THSC/TREX-2 subunits Thp1, Sac3 and Sus1 |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcription is a major obstacle for replication fork progression and a cause of genome instability. Such instability increases in mutants with a suboptimal assembly of the nascent messenger ribonucleo-protein particle (mRNP), as THO/TREX and the NPC-associated THSC/TREX-2 complex.
We used microarrays to analyze the global impact of THSC/TREX-2 in gene expression and found that Thp1 and Sac3 depletion has a functional impact in highly-expressed, long and G+C-rich genes regardless of their function
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| Overall design |
S. cerevisiae strains were grown in YPD liquid culture, total RNA was isolated and hybridized on Affymetrix microarrays
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| Contributor(s) |
Santos-Pereira JM, García-Rubio ML, González-Aguilera C, Luna R, Aguilera A |
| Citation(s) |
25294824 |
| |
| Submission date |
Apr 10, 2014 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Andrés Aguilera |
| Organization name |
CABIMER, Universidad de Sevilla
|
| Department |
Genome BIology
|
| Lab |
Genome Instability & Cancer
|
| Street address |
Av. Américo Vespucio 24
|
| City |
Seville |
| ZIP/Postal code |
41092 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
|
| Samples (15)
|
| GSM1367034 |
BY4742, biological rep. 1 |
| GSM1367035 |
BY4742, biological rep. 2 |
| GSM1367036 |
BY4742, biological rep. 3 |
| GSM1367037 |
sac3∆, biological rep. 1 |
| GSM1367038 |
sac3∆, biological rep. 2 |
| GSM1367039 |
sac3∆, biological rep. 3 |
| GSM1367040 |
sus1∆, biological rep. 1 |
| GSM1367041 |
sus1∆, biological rep. 2 |
| GSM1367042 |
sus1∆, biological rep. 3 |
| GSM1367043 |
thp1∆, biological rep. 1 |
| GSM1367044 |
thp1∆, biological rep. 2 |
| GSM1367045 |
thp1∆, biological rep. 3 |
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| This SubSeries is part of SuperSeries: |
| GSE56703 |
Microarray and ChIP-chip analyses of the THSC/TREX-2 complex |
|
| Relations |
| BioProject |
PRJNA244325 |
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