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| Status |
Public on Dec 31, 2014 |
| Title |
Expression data from hsa-mir-125 transfected MCF7 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
MicroRNA (miRNA) deregulation has long been associated with various cancers. Among an expanding list of cancer related miRNAs, deregulation of miR-125b has been well documented in various cancers including breast. Based on current knowledge, miR-125b is considered to be a tumor suppressor in breast cancers. While important mRNA targets have been defined for miR-125b, here, we aimed to further investigate direct/indirect consequences of miR-125b expression in breast cancer cells by using a transcriptome approach. Upon miR-125b expression, a total of 140 cancer related genes were found to be differentially expressed in breast cancer cells.
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| Overall design |
MCF7 cells were stably transfected with pSUPER-mir-125b and pSUPER empty vector controls. Polyclones were selected by G418, molecular confirmation of mir-125b expression was completed by TaqMan RT-qPCR assays.
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| Contributor(s) |
Akman HB, Selcuklu SD, Donoghue MT, Akhavantabasi S, Sapmaz A, Spillane C, Yakicier MC, Erson-Bensan AE |
| Citation(s) |
25539763 |
| |
| Submission date |
May 13, 2014 |
| Last update date |
Jul 08, 2016 |
| Contact name |
Ayse Elif Erson-Bensan |
| E-mail(s) |
erson@metu.edu.tr
|
| Organization name |
METU
|
| Department |
Biology
|
| Lab |
Molecular Cancer Biology
|
| Street address |
Universiteler Mah. No:1
|
| City |
Ankara |
| State/province |
Cankaya |
| ZIP/Postal code |
06800 |
| Country |
Turkey |
| |
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| Platforms (1) |
| GPL201 |
[HG-Focus] Affymetrix Human HG-Focus Target Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA247354 |
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