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Status |
Public on Dec 31, 2014 |
Title |
Expression data from hsa-mir-125 transfected MCF7 cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
MicroRNA (miRNA) deregulation has long been associated with various cancers. Among an expanding list of cancer related miRNAs, deregulation of miR-125b has been well documented in various cancers including breast. Based on current knowledge, miR-125b is considered to be a tumor suppressor in breast cancers. While important mRNA targets have been defined for miR-125b, here, we aimed to further investigate direct/indirect consequences of miR-125b expression in breast cancer cells by using a transcriptome approach. Upon miR-125b expression, a total of 140 cancer related genes were found to be differentially expressed in breast cancer cells.
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Overall design |
MCF7 cells were stably transfected with pSUPER-mir-125b and pSUPER empty vector controls. Polyclones were selected by G418, molecular confirmation of mir-125b expression was completed by TaqMan RT-qPCR assays.
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Contributor(s) |
Akman HB, Selcuklu SD, Donoghue MT, Akhavantabasi S, Sapmaz A, Spillane C, Yakicier MC, Erson-Bensan AE |
Citation(s) |
25539763 |
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Submission date |
May 13, 2014 |
Last update date |
Jul 08, 2016 |
Contact name |
Ayse Elif Erson-Bensan |
E-mail(s) |
erson@metu.edu.tr
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Organization name |
METU
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Department |
Biology
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Lab |
Molecular Cancer Biology
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Street address |
Universiteler Mah. No:1
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City |
Ankara |
State/province |
Cankaya |
ZIP/Postal code |
06800 |
Country |
Turkey |
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Platforms (1) |
GPL201 |
[HG-Focus] Affymetrix Human HG-Focus Target Array |
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Samples (9)
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Relations |
BioProject |
PRJNA247354 |