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| Status |
Public on Dec 31, 2022 |
| Title |
Expression data form PI3K Knockout colon cancer cell lines (HCT116 and DLD1) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Whole transciptome analysis of colon cancer mutated cell lines(HCT116 and DLD1) under serum starvation conditions (19hrs-0.5%FBS)
We used microarrays to compare gene regulation of truncated cell lines, knocked-out of either the wild type or mutated allele of PI3K, for two independent colon cancer cell lines
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| Overall design |
Vogelstein's lab generated two truncated cell lines, knocked-out of either the wild type or mutated allele of PI3K, for two independent colon cancer cell liness (HCT116 and DLD1). These 4 cell lines were selected for RNA extraction under serum starvation conditions and RNA was used for hybridization on Affymetrix microarrays. We sought to identify potent regulators of carcinogenesis and metastases under these conditions
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| Contributor(s) |
Kyrkou A, Murphy C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 27, 2014 |
| Last update date |
Dec 31, 2022 |
| Contact name |
Carol Murphy |
| E-mail(s) |
cmurphy@cc.uoi.gr
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| Phone |
+30-2651007815
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| Organization name |
FORTH-IMBB
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| Department |
Biomedical reseach
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| Street address |
Panepistimioupoli
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| City |
Ioannina |
| ZIP/Postal code |
45110 |
| Country |
Greece |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| GSM1422130 |
HCT116 line knocked-out for the mutated H1047R allele (expression wild type allele alone), biological rep1 |
| GSM1422131 |
HCT116 line knocked-out for the mutated H1047R allele (expression wild type allele alone), biological rep2 |
| GSM1422132 |
HCT116 line knocked-out for the mutated H1047R allele (expression wild type allele alone), biological rep3 |
| GSM1422133 |
HCT116 line knocked-out for the wild type allele (expression of H1047R mutated allele alone), biological rep1 |
| GSM1422134 |
HCT116 line knocked-out for the wild type allele (expression of H1047R mutated allele alone), biological rep2 |
| GSM1422135 |
HCT116 line knocked-out for the wild type allele (expression of H1047R mutated allele alone), biological rep3 |
| GSM1422136 |
DLD1 line knocked-out for the mutated E545K allele (expression wild type allele alone), biological rep1 |
| GSM1422137 |
DLD1 line knocked-out for the mutated E545K allele (expression wild type allele alone), biological rep2 |
| GSM1422138 |
DLD1 line knocked-out for the mutated E545K allele (expression wild type allele alone), biological rep3 |
| GSM1422139 |
DLD1 line knocked-out for the wild type allele (expression of E545K mutated allele alone), biological rep1 |
| GSM1422140 |
DLD1 line knocked-out for the wild type allele (expression of E545K mutated allele alone), biological rep2 |
| GSM1422141 |
DLD1 line knocked-out for the wild type allele (expression of E545K mutated allele alone), biological rep3 |
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| Relations |
| BioProject |
PRJNA253944 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE58904_RAW.tar |
52.6 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE58904_processed_350vs349_FC_2_p_0.05.txt.gz |
2.8 Kb |
(ftp)(http) |
TXT |
| GSE58904_processed_353vs351_FC_2_p_0.05.txt.gz |
3.4 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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