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| Status |
Public on Sep 01, 2014 |
| Title |
Chronic Bisphenol A and Genistein Exposure in MCF7 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Exposures to environmental endocrine disruptors is growing and human contact in industrialized countries has become signficant and constant. We studied the effect of chronic exposure to two endocrine disrupting compound, bisphenol A and genistein, in an in vitro cell culture system. MCF7 cells were cultured for greater than 70 passages under normal (MCF7-F) conditions or with the addition of 50 nM BPA (MCF7-B) or GEN (MCF7-G). We performed transcriptome analysis of the all three cell lines in the absence of any estrogenic compounds and in the presence of 10 nM estradiol for 3 hours.
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| Overall design |
The MCF7-F, B, G cell lines were starved of all estrogenic compounds by culturing the cells for 72 hours in phenol red free DMEM containing 5% charcoal/dextran treated FBS. Cells were then treated with either ethanol vehicle or 10 nM 17b-estradiol for 3 hours. Total RNA was harvested and utilized for whole genome analysis on the Affymetrix Human Trascriptome Array 2.0. The experiment was performed in duplicate
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| Contributor(s) |
Burd CJ, Patterson AR, Mo X |
| Citation(s) |
25594248 |
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| Submission date |
Jul 11, 2014 |
| Last update date |
Oct 17, 2019 |
| Contact name |
Craig Joseph Burd |
| E-mail(s) |
craig.burd@osumc.edu
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| Phone |
614-688-7458
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| Organization name |
The Ohio State University
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| Department |
Molecular Genetics
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| Street address |
460 W. 12th Ave. Rm 920 BRT
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| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (12)
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| GSM1435449 |
MCF7-F, estradiol treated, replicate 2 |
| GSM1435450 |
MCF7-B, ethanol treated, replicate 1 |
| GSM1435451 |
MCF7-B, ethanol treated, replicate 2 |
| GSM1435452 |
MCF7-B, estradiol treated, replicate 1 |
| GSM1435453 |
MCF7-B, estradiol treated, replicate 2 |
| GSM1435454 |
MCF7-G, ethanol treated, replicate 1 |
| GSM1435455 |
MCF7-G, ethanol treated, replicate 2 |
| GSM1435456 |
MCF7-G, estradiol treated, replicate 1 |
| GSM1435457 |
MCF7-G, estradiol treated, replicate 2 |
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| Relations |
| BioProject |
PRJNA255108 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE59345_RAW.tar |
300.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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