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| Status |
Public on Jul 17, 2014 |
| Title |
Expression data for miR-33a over-expression in CD133-negative D456MG cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
MiR-33a is involved in the maintenance of Glioma Initiating Cells (GIC) and tumor progression. MicroRNA-33a could promote GIC growth and self-renewal by regulating two pathways including cAMP/PKA pathway and Notch pathway. We used microarrays to identify the direct target genes of miR-33a in a glioblastoma cell line D456MG.
We used microarrays to detail the global change of gene expression after miR-33a over-expression and identified target genes during this process.
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| Overall design |
Cells were infected with lenti-virus expressing a control vector (pWPXLD) or human primary miR-33a. Then RNA was extracted and gene expression was profiled by Affymetrix microarray.
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| Contributor(s) |
Wang H, Sun T, Hu J |
| Citation(s) |
25202981 |
| |
| Submission date |
Jul 16, 2014 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Hui Wang |
| E-mail(s) |
wanghui0613@gmail.com
|
| Organization name |
Duke University
|
| Street address |
380 Research Drive
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| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27707 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (2) |
| GSM1437722 |
CD133-negative D456MG cells infected with vector control |
| GSM1437723 |
CD133-negative D456MG cells with miR-33a over-expression |
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| Relations |
| BioProject |
PRJNA255417 |
