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| Status |
Public on Oct 05, 2015 |
| Title |
Pyronema confluens circadian rhythm analysis |
| Organism |
Pyronema omphalodes CBS 100304 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The aim of this analysis was the identification of candidate genes that might be regulated in a circadian manner in the filamentous ascomycete Pyronema confluens. The fungus was grown in submerged culture to promote vegetative growth, and after a period in constant light, the samples were shifted to darkness (DD). Cultures were sampled after 24 and 36 hours in DD. The samples were used for RNA-seq analysis, and candidate genes with significantly higher expression after 24 vs. 36 hours or the reverse were further analyzed with RT-qPCR.
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| |
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| Overall design |
For each growth condition (DD24 and DD36), three independent biological replicates were performed and sequenced.
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| |
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| Contributor(s) |
Nowrousian M, Traeger S |
| Citation(s) |
26254031 |
| |
| Submission date |
Sep 09, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Minou Nowrousian |
| E-mail(s) |
minou.nowrousian@rub.de
|
| Phone |
+49 234 3224588
|
| Organization name |
Ruhr-University Bochum
|
| Department |
Molecular and Cellular Botany
|
| Street address |
Universitaetsstr. 150
|
| City |
Bochum |
| ZIP/Postal code |
44780 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL19175 |
Illumina HiSeq 2000 (Pyronema omphalodes CBS 100304) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA260620 |
| SRA |
SRP046748 |
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