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| Status |
Public on Feb 09, 2017 |
| Title |
Role of the PhoP-PhoQ Gene Regulatory System in Adaptation of Yersinia pestis to Environmental Stress in the Flea Digestive Tract |
| Platform organisms |
Borreliella burgdorferi; Yersinia pestis; Coxiella burnetii; Chlamydia trachomatis; Staphylococcus aureus; Streptococcus pyogenes |
| Sample organism |
Yersinia pestis |
| Experiment type |
Expression profiling by array
|
| Summary |
The Yersinia pestis PhoPQ gene regulatory system is induced during infection of the flea digestive tract and is required to produce adherent biofilm in the foregut, which greatly enhances bacterial transmission during a flea bite. To understand the in vivo context of PhoPQ induction and to determine PhoP-regulated targets in the flea, we undertook whole genome comparative transcriptional profiling of Y. pestis wild-type and ΔphoP strains isolated from infected fleas and from temperature-matched in vitro planktonic and flowcell biofilm cultures. In the absence of PhoP regulation, the gene expression program indicated that the bacteria experience diverse physiological stresses and are in a metabolically less active state. Multiple stress response genes, including several toxin-antitoxin loci and YhcN family genes responsible for increased acid tolerance, were upregulated in the phoP mutant during flea infection. The data imply that PhoPQ is induced by low pH in the flea gut, and that PhoP modulates physiologic adaptation to acid and other stresses encountered during infection of the flea. This adaptive response, together with PhoP-dependent modification of the bacterial outer surface that includes repression of pH 6 antigen fimbriae, supports stable biofilm development in the flea foregut.
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| Overall design |
Yersinia pestis wild type vs. phoP mutant at various growth conditions
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| Contributor(s) |
Vadyvaloo V, Viall A, Jarrett C, Hinz A, Sturdevant D, Hinnebusch J |
| Citation(s) |
25804213 |
| |
| Submission date |
Sep 18, 2014 |
| Last update date |
Oct 16, 2018 |
| Contact name |
Dan Sturdevant |
| E-mail(s) |
dsturdevant@niaid.nih.gov
|
| Phone |
4063639248
|
| Organization name |
NIH
|
| Department |
NIAID
|
| Lab |
RTS
|
| Street address |
903 S 4th street
|
| City |
Hamilton |
| State/province |
MT |
| ZIP/Postal code |
59840 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL2129 |
Affymetrix RML Custom Pathogenic chip 1 |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA261452 |
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