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Series GSE6281 Query DataSets for GSE6281
Status Public on Apr 03, 2007
Title Gene expression time-course in the human skin during elicitation of allergic contact dermatitis
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Genes involved in the inflammatory response resulting in allergic contact dermatitis (ACD) are only partly known. In this study, we introduce the use of high density oligonucleotide arrays for gene expression profiling in human skin during the elicitation of ACD. Skin biopsies from normal and nickel-exposed skin were obtained from 7 nickel-allergic patients and 5 non-allergic controls at four different time points during elicitation of eczema: 0h, 7h, 48h and 96h. Each gene expression profile was analysed by hybridization to high density oligonucletide arrays.
Cluster analysis of 74 genes found to be differentially expressed in the patients over time revealed that the patient samples may be categorised into two groups: An early time point group (0h and 7h) and a late time point group (48h and 96h). Compared to the early time points, late time point skin samples were characterised by the up-regulation of inflammatory molecules including genes involved in the class I antigen presenting pathway and genes involved in lymphocyte adhesion and motility
Correspondence analysis including both patients and controls revealed three distinct groups: i) the control group, ii) the early time point patient group (0h and 7h) and iii) the late time point patient group (48h and 96h).
Keywords: time course, allergic response
 
Overall design For the microarray study, 7 nickel-allergic patients and 5 non-allergic controls were recruited. All subjects were female. The skin covering the upper nates was exposed to nickel delivered during a patch test. Skin biopsies were taken to generate a time-series. Skin was exposed to nickel for 0h, 7h, 48h and 96h.
All nickel allergic patients reacted with visible eczama at the 48h and 96h time-points. No eczema was vissible at the 0h or 7h time-points. Control subjects did not show visible eczema at any time-point.

RNA was extracted from the skin biopsies and 34 biopsies yielded RNA of sufficient quantity and quality for micro-array analysis.
 
Contributor(s) Pedersen MB, Skov L, Menné T, Duus JJ, Olsen J
Citation(s) 17597826
Submission date Nov 15, 2006
Last update date Mar 25, 2019
Contact name Jorgen Olsen
E-mail(s) jolsen@sund.ku.dk
Phone +45 30304085
Organization name University of Copenhagen
Department Department of Cellular and Molecular Medicine
Street address Blegdamsvej 3
City Copenhagen
ZIP/Postal code DK2770
Country Denmark
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (34)
GSM144309 Skinbiopsy_7hoursnickel_controlsubject_k1
GSM144311 Skinbiopsy_48hoursnickel_controlsubject_k1
GSM144347 Skinbiopsy_96hoursnickel_controlsubject_k1
Relations
BioProject PRJNA99677

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Supplementary file Size Download File type/resource
GSE6281_RAW.tar 268.4 Mb (http)(custom) TAR (of CEL)

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