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| Status |
Public on Aug 01, 2007 |
| Title |
Histone H3 K4,36,79R 0 hr |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
Total RNA from two replicate cultures of wild-type and mutant strains was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H3 K4,36,79R mutant. Cells were grown in galactose media to give the 0 hour timepoint.
Keywords: repeat
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| Overall design |
Histone H3 K4,36,79R time course. Cells were grown in galactose media and then shifted to glucose media, which repressed the expression of wild-type histone H3, leaving the histone H3 K4,36,79R mutant as the sole source of histone H3 protein in the cell. Total RNA was isolated from replicate cultures of wild-type and mutant histone H3 at various time points after the shift to glucose media (0, 6, and 9 hours). Expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H3 K4,36,79R mutant.
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| Contributor(s) |
Rodriguez AM, Jin Y, Wyrick JJ |
| Citation(s) |
17664279 |
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| Submission date |
Nov 20, 2006 |
| Last update date |
Jul 01, 2016 |
| Contact name |
John J. Wyrick |
| E-mail(s) |
jwyrick@vetmed.wsu.edu
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| Phone |
509-335-8785
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| Organization name |
Washington State University
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| Department |
School of Molecular Biosciences
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| Street address |
Biotechnology Life Sciences 241
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| City |
Pullman |
| State/province |
WA |
| ZIP/Postal code |
99164 |
| Country |
USA |
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| Platforms (1) |
| GPL90 |
[YG_S98] Affymetrix Yeast Genome S98 Array |
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| Samples (4)
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| GSM146316 |
Wild-type for H3 K4,36,79R 0 hr replicate 1 |
| GSM146317 |
Wild-type for H3 K4,36,79R 0 hr replicate 2 |
| GSM146318 |
Mutant Histone H3 K4,36,79R 0 hr replicate 1 |
| GSM146319 |
Mutant Histone H3 K4,36,79R 0 hr replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE6331 |
Histone H3,K4,79R and H3,K4,36,79R (at 0, 6, and 9 hours) Mutations |
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| Relations |
| BioProject |
PRJNA105007 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE6326_RAW.tar |
5.6 Mb |
(http)(custom) |
TAR (of CEL) |
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