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| Status |
Public on Apr 29, 2015 |
| Title |
The kinase DYRK1A reciprocally regulates the differentiation of Th17 and regulatory T cells [array] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Using an unbiased chemical biology approach, we discover harmine as a novel regulator of Treg/Th17 differentiation. Harmine enhances Treg differentiation (working in conjunction with low levels of exogenous TGFb) and inhibits Th17 differentiation. Analysis of global gene expression of Tregs generated using low TGFb + harmine reveals significant similarity to Tregs generated using high TGFb only and suggests relevance of harmine-engaged mechanisms to IBD.
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| |
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| Overall design |
Naïve CD4+CD62L+ T cells were purified from Foxp3GFP mice either by MACS or FACS, then cultured in either low TGFb + harmine or high TGFb conditions. GFP+ Tregs were sorted by FACS at day 4.
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| |
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| Contributor(s) |
Khor B, Goel G, Xavier RJ |
| Citation(s) |
25998054 |
| |
| Submission date |
Dec 16, 2014 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Bernard Khor |
| E-mail(s) |
bkhor@partners.org
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Pathology
|
| Lab |
Xavier
|
| Street address |
185 Cambridge St MCPZN 7106
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE67961 |
The kinase DYRK1A reciprocally regulates the differentiation of Th17 and regulatory T cells |
|
| Relations |
| BioProject |
PRJNA270532 |
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