|
| Status |
Public on Mar 18, 2015 |
| Title |
Comparative genomics reveals Chd1 as a determinant of nucleosome spacing in vivo |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We generated S.cerevisiae strains in which endogenous copies of candidate nucleosome spacing factors were replaced with the K.lactis copies. With this candidate approach, we found that K.lactis Chd1 directed longer nucleosome repeat length in S.cerevisiae. Generating chimeric proteins revealed that the strongest contribution to this differential spacing lies in the undercharacterised N-terminus of Chd1.
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| |
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| Overall design |
nucleosome mapping for S.cerevisiae hho1, ioc3isw1, and chd1 deletion mutants and complemented with the K.lactis copies
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| |
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| Contributor(s) |
Rando OJ, Hughes AL |
| Citation(s) |
26175451 |
| |
| Submission date |
Mar 17, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Oliver Rando |
| E-mail(s) |
Oliver.Rando@umassmed.edu
|
| Phone |
508-856-8879
|
| Organization name |
UMass Medical School
|
| Street address |
364 Plantation St.
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605-4321 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL13272 |
Illumina Genome Analyzer IIx (Saccharomyces cerevisiae) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
| GPL17143 |
Illumina MiSeq (Saccharomyces cerevisiae) |
|
| Samples (22)
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| Relations |
| BioProject |
PRJNA278642 |
| SRA |
SRP056242 |
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