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| Status |
Public on Nov 02, 2015 |
| Title |
Different exercise modalities have distinct effects on the transcriptome profile in the male rat femoral diaphysis |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mechanical loading is a key determinant of bone mass, geometry and strength and is essential to maintain optimal skeletal health. We hypothesized that spontaneous low-impact exercise would affect global gene transcript levels in cortical bone of growing rats. We used RNA-Seq to analyse the transcriptome of the femoral mid-diaphysis in pre-pubertal male rats that were assigned to one of three exercise groups for 15 days: control (CON); bipedal stance (BPS); and wheel exercise (WEX). RNA-seq analysis identified 808 and 324 differentially expressed transcripts in the BPS and WEX animals, respectively. In WEX animals, the up-regulated transcripts were enriched for gene ontology terms associated with bone metabolism. Both BPS and WEX animals showed changes in transcripts that were enriched for muscle-related processes. However, in WEX these transcripts were down-regulated while in BPS such transcripts were up-regulated. Importantly, we observed that the exercise mode had diametrically opposite effects on transcripts for multiple genes within the integrin-linked kinase (ILK) and Ca2+ signalling pathways such that they were up-regulated in BPS and down-regulated in WEX. The findings are important for our understanding of possible ways in which different exercise regimens might affect bone when normal activities apply mechanical stimuli during post-natal growth and development.
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| Overall design |
24 weanling (21 day old) male Sprague-Dawley rats were assigned to three exercise groups .After the 15 day trial period, total RNA was extracted from the femoral diaphysis and mRNA profiles were analysed using RNA-seq
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| Contributor(s) |
Sontam DM, Firth EC, Tsai P, Vickers MH, O'Sullivan JM |
| Citation(s) |
26471755 |
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| Submission date |
Apr 13, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Justin M. O'Sullivan |
| E-mail(s) |
justin.osullivan@auckland.ac.nz
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| Organization name |
University of Auckland
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| Department |
Liggins Institute
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| Street address |
85 Park Road, Grafton
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| City |
Auckland |
| ZIP/Postal code |
1023 |
| Country |
New Zealand |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA280937 |
| SRA |
SRP057120 |
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