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| Status |
Public on Sep 30, 2015 |
| Title |
Expression data from Dicer and Ago2-knockdown HeLa cells |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Dicer and Argonaute2 (Ago2) gene is involving in microRNA (miRNA) maturation. Knockdown of these genes has great impact on miRNA expression profiles.
We used microarrays to detail the miRNA expression profiles in Dicer- and Ago2-knockdown HeLa cells and demonstarted that the significant difference between Ago2-knockdown and Dicer- and Ago2-co-knockdown HeLa cells were not found.
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| Overall design |
Hela-shDicer cells, which inducibly express shDicer by Dox treatment, were cultured in Dox-containing medium for 48 h, followed by transfection with siControl or siAgo2. After 48 h incubation, the cells were collected and the miRNA expression profiles were in the cells analyzed by the miRNA microarray analysis.
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| Contributor(s) |
Machitani M, Sakurai F, Mizuguchi H |
| Citation(s) |
27273616 |
| |
| Submission date |
Apr 17, 2015 |
| Last update date |
May 02, 2017 |
| Contact name |
Mitsuhiro Machitani |
| E-mail(s) |
mmachita@ncc.go.jp
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| Phone |
081-3-3547-5201
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| Organization name |
National Cancer Center Research Institute
|
| Lab |
Division of Cancer Stem Cell
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| Street address |
5-1-1 Tsukiji
|
| City |
Chuo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
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| Platforms (1) |
| GPL19117 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA281458 |
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