|
| Status |
Public on Jun 21, 2007 |
| Title |
Gingival Epithelial Cell Transcriptional Responses to Commensal and Opportunistic Oral Microbial Species. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptional profiling was utilized to define the biological pathways of gingival epithelial cells modulated by co-culture with the oral commensal S. gordonii and the opportunistic commensal F. nucleatum.
We used microarrays to detail the global programme of gene expression underlying infection and identified distinct classes of up- and down-regulated genes during this process.
Keywords: infection state
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| |
|
| Overall design |
Gingival epithelial HIGK cells were sham infected (CTRL) and infected with either the oral commensal S. gordonii (Sg) or the opportunistic commensal F. nucleatum (Fn). These samples were hybridized to Affymetrix microarrays. Understanding how host cells have adapted to commensals, and how barrier cells respond to limit their impact, provides a mechanistic biological basis of health in the mixed bacterial-human ecosystem of the oral cavity.
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| |
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| Contributor(s) |
Handfield M, Lamont RJ, Hasegawa Y, Mans JJ, Baker HV |
| Citation(s) |
17307939 |
| |
| Submission date |
Jan 31, 2007 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Martin Handfield |
| E-mail(s) |
mhandfield@dental.ufl.edu
|
| Phone |
352-846-0763
|
| Organization name |
University of Florida
|
| Department |
Oral Biology
|
| Lab |
Handfield Lab
|
| Street address |
1600 SW Archer Road, BOX 100424
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610-0424 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
|
| Samples (12)
|
| GSM159477 |
HIGK cells infected with Streptococcus gordonii, biological rep1 |
| GSM159478 |
HIGK cells infected with Streptococcus gordonii, biological rep2 |
| GSM159479 |
HIGK cells infected with Streptococcus gordonii, biological rep3 |
| GSM159480 |
HIGK cells infected with Streptococcus gordonii, biological rep4 |
| GSM159481 |
HIGK cells infected with Fusobacterium nucleatum, biological rep1 |
| GSM159482 |
HIGK cells infected with Fusobacterium nucleatum, biological rep2 |
| GSM159483 |
HIGK cells infected with Fusobacterium nucleatum, biological rep3 |
| GSM159484 |
HIGK cells infected with Fusobacterium nucleatum, biological rep4 |
| GSM159485 |
Sham infected HIGK cells, biological rep1 |
| GSM159486 |
Sham infected HIGK cells, biological rep2 |
| GSM159487 |
Sham infected HIGK cells, biological rep3 |
| GSM159488 |
Sham infected HIGK cells, biological rep4 |
|
| Relations |
| BioProject |
PRJNA99353 |
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