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| Status |
Public on Mar 12, 2016 |
| Title |
Sequence-Targeted Nucleosome Sliding in vivo - Nucleosome Mapping |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Nucleosome positions were determined in wild type cells, cells lacking Isw2 or Ume6, and cells containing a hybrid Chd1-Ume6 chimeric remodeler
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| Overall design |
Matched MNase digests from W303 strain variants during log growth (OD600=0.4-0.6) were subject to paired-end sequencing for nucleosome mapping. For effects of the engineered fusion remodeling protein, a catalytically inactive (ATPase dead D513N) variant was included as a control.
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| |
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| Contributor(s) |
McKnight JN, Tsukiyama T, Bowman GD |
| Citation(s) |
26993344 |
| |
| Submission date |
Sep 01, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Jeffrey N McKnight |
| E-mail(s) |
jmcknig2@uoregon.edu
|
| Organization name |
University of Oregon
|
| Department |
Institute of Molecular Biology
|
| Lab |
McKnight Lab
|
| Street address |
1229 University of Oregon, 1318 Franklin Blvd, Rm 273 Onyx Bridge
|
| City |
Eugene |
| State/province |
OR |
| ZIP/Postal code |
97408 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9134 |
Illumina Genome Analyzer (Saccharomyces cerevisiae) |
|
| Samples (13)
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| This SubSeries is part of SuperSeries: |
| GSE72572 |
Sequence-Targeted Nucleosome Sliding in vivo |
|
| Relations |
| BioProject |
PRJNA294376 |
| SRA |
SRP063046 |
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