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| Status |
Public on Nov 06, 2015 |
| Title |
On the relationship of protein and mRNA dynamics in vertebrate embryonic development [polyA] |
| Organism |
Xenopus laevis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
A biochemical explanation of development from the fertilized egg to the adult anatomy requires an understanding of the complement of proteins and RNAs expressed over time during embryogenesis. Here we present a comprehensive characterization of protein and mRNA dynamics across early development in Xenopus. Surprisingly, we find that most proteins change very little and duplicated genes are expressed similarly. While the correlation between mRNA levels and protein expression is poor, a mass action kinetics model parametrized by the protein synthesis and degradation rates regresses protein dynamics to RNA dynamics, corrected for initial protein concentration. This study provides a rich resource for developmental biologists, unveiling detailed data for absolute levels of ~10K proteins and ~28K transcripts via convenient web portal. It underscores the lasting impact of maternal dowry, finds surprisingly few cases where degradation alone drives a change in protein level, and highlights the importance of transcription in shaping the proteome.
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| Overall design |
mRNA from 18 samples each at a different developmental stage. Libraries were constructed using RNA enriched for mRNA by Poly(A) extraction using Dynabeads (invitrogen) and the EpiCenter ScripSeq kit V1 using 50-300ng input RNA, and 10 cycles amplification.
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| Contributor(s) |
Peshkin L, Wuhr M, Pearl EJ, Haas W, Freeman RM, Gerhart JC, Klein AM, Horb ME, Gygi SP, Kirscher MW |
| Citation(s) |
26555057 |
| |
| Submission date |
Oct 08, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Esther Jane Pearl |
| Organization name |
Marine Biological Laboratory
|
| Department |
National Xenopus Resource
|
| Street address |
7 MBL Street
|
| City |
Woods Hole |
| State/province |
MA |
| ZIP/Postal code |
02543 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20998 |
Illumina HiSeq 1000 (Xenopus laevis) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE73905 |
On the relationship of protein and mRNA dynamics in vertebrate embryonic development |
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| Relations |
| BioProject |
PRJNA298254 |
| SRA |
SRP064629 |
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