|
| Status |
Public on Jan 01, 2016 |
| Title |
Transcriptome profiling of post-mature green seeds from Arabidopsis ddcc mutant and wild-type |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The role of on-CG methylation in seed development and dormancy remains unknown. There are four genes in charge of non-CG methylation in Arabidopsis: drm1, drm2, cmt2 and cmt3. The majority of non-CG methylation in vegetative tissues, leaf, is gone in homozygous ddcc mutant line (Hume et al., 2014). To uncover the possible role of non-CG DNA methylation in seed development and dormancy, we characterized the transcriptome of ddcc mutant in Arabidopsis post-mature green seeds using Illumina sequencing. Meanwhile, post-mature green seeds from wild type were used as control.
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| |
|
| Overall design |
Illumina sequencing of transcripts from post-mature green seeds of ddcc mutant and wild type. Two biological replicates were collected.
|
| |
|
| Contributor(s) |
Goldberg RB, Lin J, Chen M |
| Citation(s) |
29078418 |
| |
| Submission date |
Dec 31, 2015 |
| Last update date |
Jul 19, 2019 |
| Contact name |
Bob Goldberg |
| E-mail(s) |
bobglab@mcdb.ucla.edu
|
| Phone |
310-825-3270
|
| Organization name |
University of California, Los Angeles
|
| Department |
Molecular, Cell and Developmental Biology
|
| Street address |
610 Charles E Young Drive East
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
|
| Samples (4)
|
| GSM2024779 |
ddcc mutant post-mature green seeds biological replicate 1 |
| GSM2024780 |
ddcc mutant post-mature green seeds biological replicate 2 |
| GSM2024781 |
wild type post-mature green seeds biological replicate 1 |
| GSM2024782 |
wild type post-mature green seeds biological replicate 2 |
|
| Relations |
| BioProject |
PRJNA307336 |
| SRA |
SRP067963 |
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