|
| Status |
Public on Feb 29, 2016 |
| Title |
Human hematopoietic leukemic k562 cells: control Vs CXCR4 constitutively active mutant cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The low homing and engraftment efficiency especially in context of low availability of donor CD34+ HSPCs is a major challenge, which limits the clinical applications of HSC therapies. SDF-1/CXCR4 axis plays a principle role in the homing and engraftment of hematopoietic stem/progenitor cells. Activation of CXCR4 induces cell trafficking and homing to the marrow microenvironment, where it retains hematopoietic stem cells in close contact with marrow stromal cells. The aim of our study is to understand the mechanisms of other molecules acting along with CXCR4 to provide new insights in the regulation of cell phenotypes. This data shows the transcriptional profiling of human hematopoetic leukemic k562 cells comparing control, wild type and two different mutant cells where mutant cells have constitutively activated CXCR4 gene expression where mutant 1 and 2 have different aminoacid substituions.
|
| |
|
| Overall design |
biological replicates: three control, three wild type, three mutant 1, three mutant 2
|
| |
|
| Contributor(s) |
Gangenahalli G, Sharma M, Kaur R |
| Citation(s) |
24693205 |
| |
| Submission date |
Jan 05, 2016 |
| Last update date |
Aug 11, 2016 |
| Contact name |
Gurudutta U Gangenahalli |
| E-mail(s) |
gugdutta@rediffmail.com
|
| Phone |
9101123905144
|
| Organization name |
Institute of Nuclear Medicine & Allied Sciences
|
| Department |
Stem Cell Research Division
|
| Lab |
Stem Cell & Gene Therapy Research Group
|
| Street address |
Brig. S. K. Mazumdar Marg, Timarpur
|
| City |
Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110054 |
| Country |
India |
| |
|
| Platforms (1) |
| GPL13252 |
Agilent-027114 Genotypic Technology designed Custom Human Whole Genome 8x60k Microarray |
|
| Samples (12)
|
| GSM2027678 |
control human hematopoietic leukemic k562 cells replicate 1 |
| GSM2027679 |
control human hematopoietic leukemic k562 cells replicate 2 |
| GSM2027680 |
control human hematopoietic leukemic k562 cells replicate 3 |
| GSM2027681 |
wild type CXCR4 transfected human hematopoietic leukemic k562 cells replicate 1 |
| GSM2027682 |
wild type CXCR4 transfected human hematopoietic leukemic k562 cells replicate 2 |
| GSM2027683 |
wild type CXCR4 transfected human hematopoietic leukemic k562 cells replicate 3 |
| GSM2027684 |
mutant 1 CXCR4 transfected human hematopoetic leukemic k562 cells replicate 1 |
| GSM2027685 |
mutant 1 CXCR4 transfected human hematopoetic leukemic k562 cells replicate 2 |
| GSM2027686 |
mutant 1 CXCR4 transfected human hematopoetic leukemic k562 cells replicate 3 |
| GSM2027687 |
mutant 2 CXCR4 transfected human hematopoetic leukemic k562 cells replicate 1 |
| GSM2027688 |
mutant 2 CXCR4 transfected human hematopoetic leukemic k562 cells replicate 2 |
| GSM2027689 |
mutant 2 CXCR4 transfected human hematopoetic leukemic k562 cells replicate 3 |
|
| Relations |
| BioProject |
PRJNA307693 |
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