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| Status |
Public on Jan 12, 2019 |
| Title |
Knockdown of Brd4 or SEC affects the HMBA-induced global Pol II pausing release |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To test whether Brd4 and SEC co-regulate the release of promoter-proximally paused Pol II, we performed Pol II ChIP-Seq to analyze the effect of depletion of Brd4 or SEC on HMBA-induced pause release in HCT116 cells.
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| |
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| Overall design |
Total 6 ChIP-seq samples were sequenced. Samples with indicated shRNA(s) infection in HCT116 cells were treated with solvent or 10 mM HMBA for 2hr. Signal from input sample was considered as the background signal to be substracted from all ChIP-seq samples.The number of ChIP-seq reads from each ChIP-seq sample were normalized by the concentration of input DNA and then calculated Pol II pause ratio (TR).
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| |
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| Contributor(s) |
Liu M, Yu B |
| Citation(s) |
27353326 |
| |
| Submission date |
Jan 12, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Min Liu |
| E-mail(s) |
minliu@xmu.edu.cn
|
| Phone |
86-592-2184533
|
| Organization name |
Xiamen University
|
| Department |
School of Life Sciences
|
| Lab |
State Key Laboratory of Cellular Stress Biology
|
| Street address |
Rm.214, Bldg. Chaoyang Huang, Xiamen University-Xiang'an Campus
|
| City |
Xiamen |
| State/province |
Fujian |
| ZIP/Postal code |
361101 |
| Country |
China |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA308577 |
| SRA |
SRP068363 |
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