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| Status |
Public on Apr 25, 2016 |
| Title |
Genome-wide binding pattern of β-catenin during Xenopus gastrulation |
| Organism |
Xenopus laevis |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The canonical Wnt/β-catenin signaling pathway plays multiple roles during Xenopus gastrulation, including posteriorization of the neural plate, patterning of the mesoderm, and induction of the neural crest. Wnt signaling stabilizes β-catenin, which then activates target genes. However, few targets of this signaling pathway that mediate early developmental processes are known. Here we sought to identify transcriptional targets of the Wnt/β-catenin signaling pathway using a genome-wide approach.
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| Overall design |
Xenopus laevis emrbyos were injected with a triple FLAG tagged beta-catenin at levels that did not effect phenotype. Pools of about 500 embryos were fixed for ChIP processing. An input sample was taken prior to immunoprecipitation with a Flag antibody. Both input and ChIP material were used to make sequencing libraries.
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| |
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| Contributor(s) |
Kjolby RS, Harland RM |
| Citation(s) |
27091726 |
| |
| Submission date |
Jan 28, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Richard Harland |
| Organization name |
University of California, Berkeley
|
| Street address |
Life Sciences Addition #3200
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17682 |
Illumina HiSeq 2000 (Xenopus laevis) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE77365 |
Wnt/β-catenin in Xenopus laevis gastrulation |
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| Relations |
| BioProject |
PRJNA310141 |
| SRA |
SRP069034 |
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