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| Status |
Public on Feb 09, 2017 |
| Title |
Identifying genes differentially expressed in MDCK cells with scribble knock-down (scribKD) |
| Organism |
Canis lupus familiaris |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Aim: To identify genes and pathways involved in elimination of scribKD cells by competition.
Methods: We carried out mRNA sequencing of MDCK-II cells carrying a tetracycline-inducible scribble shRNA construct.
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| Overall design |
We compared the transcriptomes of MDCK cells with scribble knock-down (scribKD+Tet) to that of control MDCK cells with wild-type scribble expression (scribKD-Tet) and to that of an isolate of scribKD cells that is resistant to cell competition (scribRES+Tet). The samples were obtained across at least two independent experiments.
Each experiment contained at least two replicates per condition.
mRNA profiles of three populations of MDCK-II cells were obtained by Illumina High-Throughput sequencing.
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| Contributor(s) |
Goschorska M, Piddini E |
| Citation(s) |
27109213 |
| |
| Submission date |
Mar 09, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Charles Bradshaw |
| Organization name |
University of Cambridge
|
| Department |
Gurdon Institute
|
| Street address |
Tennis Court Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 1QN |
| Country |
United Kingdom |
| |
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| Platforms (2) |
| GPL16540 |
Illumina HiSeq 2000 (Canis lupus familiaris) |
| GPL20988 |
Illumina HiSeq 2500 (Canis lupus familiaris) |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA314784 |
| SRA |
SRP071342 |
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