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| Status |
Public on Feb 22, 2018 |
| Title |
Somatic PRDM2 c.4459delA mutations in colorectal cancers control histone methylation and tumor growth |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To identify the contribution of PRDM2 c.4459delA mutation to colorectal tumorigenesis
Methods: We employed rAAV-mediated genome editing to correct somatic PRDM2 c.4459delA mutation in homozygously mutated cell line. Using next-generation sequencing we have compared transcriptional profile of parental and PRDM2-corrected cells.
Results: RNA-seq profiling revealed that several hallmark cancer gene sets are affected by PRDM2 c.4459delA
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| Overall design |
To address if the PRDM2 wt cells were transcriptionally different at a global level compared to their parental cells that carry PRDM2 c.4459delA mutation, we performed RNA-sequencing of parental cells and three independently generated clones with corrected PRDM2. RNA-seq profile was generated for cells grown under normal culture conditions (10% FBS) as well as reduced serum conditions (0.5% FBS).
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| Contributor(s) |
Pandzic T, Sjöblom T |
| Citation(s) |
29228717 |
| |
| Submission date |
Apr 07, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
L. H. |
| Organization name |
Uppsala University
|
| Street address |
Dag Hammarskjölds Väg 20
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| City |
Uppsala |
| ZIP/Postal code |
751 85 |
| Country |
Sweden |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA317667 |
| SRA |
SRP072990 |
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