|
| Status |
Public on May 12, 2016 |
| Title |
Global Mapping of Human RNA-RNA Interactions |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
We describe a new method enabling the global-scale mapping of RNA-RNA duplexes cross-linked in vivo, ‘LIGation of interacting RNA followed by high-throughput Sequencing’ (LIGR-Seq). Applying this method in human cells reveals a remarkable landscape of new RNA-RNA interactions involving all major classes of ncRNA, and mRNA. LIGR-Seq data reveal unexpected interactions between small nucleolar (sno)RNAs and mRNAs, including interactions involving the orphan C/D box snoRNA, SNORD83B, that control steady-state levels of its target mRNAs. LIGR-Seq thus represents a powerful approach for illuminating the functions of the myriad of uncharacterized RNAs that act via base-pairing interactions.
|
| |
|
| Overall design |
RNA-RNA interaction discovery in HEK293T cells in two replicates.
|
| |
|
| Contributor(s) |
Sharma E, Sterne-Weiler TH, O' Hanlon D, Blencowe BJ |
| Citation(s) |
27184080 |
| |
| Submission date |
Apr 12, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Timothy Sterne-Weiler |
| E-mail(s) |
tim.sterne.weiler@utoronto.ca
|
| Organization name |
University of Toronto
|
| Department |
The Donnelly Centre
|
| Lab |
Blencowe Lab
|
| Street address |
160 College Street, Room 1030
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5S 3E1 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA318165 |
| SRA |
SRP073158 |
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