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| Status |
Public on May 01, 2017 |
| Title |
Mfd alters global transcription patterns in undamaged Escherichia coli cells |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We uncovered evidence for an expanded role of transcription-coupled repair factor Mfd in Escherichia coli. In mfd knockout cells, expression of 75 genes was significantly increased while that of 18 genes was diminished. GO term analysis revealed that the affected genes were disproportionately from certain functional groups. RNA levels in the knockout deviated from wild type beginning at transcription start sites, arguing against premature termination as the cause. RNA-seq analysis in cells overexpressing Mfd revealed dramatic deviation of gene expression in the chromosome terminus region. Taken together, our data support a novel role for Mfd in transcription regulation.
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| |
|
| Overall design |
Examination of E coli mfd knockdown and Mfd-overexpressing cells compared to wildtype E. coli controls
|
| |
|
| Contributor(s) |
Krasich R, Kreuzer KN |
| Citation missing |
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| |
| Submission date |
May 18, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Rachel Krasich |
| Organization name |
NIEHS
|
| Street address |
111 TW Alexander Dr
|
| City |
Research Triangle Park |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL14548 |
Illumina HiSeq 2000 (Escherichia coli) |
|
| Samples (10)
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|
| Relations |
| BioProject |
PRJNA322690 |
| SRA |
SRP075662 |
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