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| Status |
Public on Dec 01, 2016 |
| Title |
Beta-catenin-regulated genes in pancreatic cancer cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Activation of the canonical Wnt signaling pathway is commonly observed in pancreatic cancer. We therefore sought to identify a gene expression profile associated with the activation of this pathway in pancreatic cancer cells.
We used microarrays to identify differentially expressed genes upon expression of an activated form of beta-catenin or its negative regulator ICAT (beta-catenin-interacting protein 1).
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| |
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| Overall design |
Pancreatic cancer cells stably transfected to express beta-catenin or ICAT were grown in DMEM tissue culture medium containing 10% FBS. Cells were grown in monolayer culture prior to RNA extraction and hybridization on Affymetrix microarrays. Three independent samples of each genotype were analyzed.
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| |
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| Contributor(s) |
Lewis BC |
| Citation(s) |
27889647 |
| |
| Submission date |
Jun 09, 2016 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Brian Lewis |
| E-mail(s) |
BRIAN.LEWIS@UMASSMED.EDU
|
| Organization name |
University of Massachusetts Medical School
|
| Department |
Molecular, Cell and Cancer Biology
|
| Street address |
364 Plantation Street, LRB 521
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA325277 |
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